Claude Generated 318,484 Scorpion Toxin Protein Designs, With 17 Lab-Validated Binders

Описание и применение

Claude создал 318 484 кандидата на мини‑биндеры для пяти скопперов, из которых 211 прошли лабораторную проверку, и 17 показали высокую аффинность, подтверждая потенциал AI‑поддерживаемого протеин‑дизайна.

Задача

How to accelerate protein design for scorpion toxin binders while ensuring experimental validation.

Результат

17 high‑affinity binders were experimentally validated from a pool of 318,484 AI‑generated candidates, demonstrating the feasibility of AI‑assisted high‑throughput protein design.

Рабочий процесс

• 1. Claude generated 318,484 minibinder candidates in 64 h.
• 2. Researchers selected 180 top designs and added 31 more, totaling 211 for testing.
• 3. Two CROs (Adaptyv Bio and Twist Bioscience) expressed candidates via cell‑free and Fc‑tagged mammalian systems.
• 4. Binding assays measured KD ≤1 µM and no off‑target CLEC12A binding.
• 5. 17 designs met all criteria for individual toxins.
• 6. Data and designs were released publicly for inspection.

Доказательства

Claude generated 318,484 candidate minibinders in 64 hours

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211 designs tested

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17 binders

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binding in the single‑digit nanomolar KD range

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