Claude Generated 318,484 Scorpion Toxin Protein Designs, With 17 Lab-Validated Binders

说明与用途

Claude 生成 318,484 个蝎子毒素结合体候选物，211 个被实验室测试，17 个显示高亲和力，证明 AI 支持的高通量蛋白设计的潜力。

问题

How to accelerate protein design for scorpion toxin binders while ensuring experimental validation.

结果

17 high‑affinity binders were experimentally validated from a pool of 318,484 AI‑generated candidates, demonstrating the feasibility of AI‑assisted high‑throughput protein design.

工作流

• 1. Claude generated 318,484 minibinder candidates in 64 h.
• 2. Researchers selected 180 top designs and added 31 more, totaling 211 for testing.
• 3. Two CROs (Adaptyv Bio and Twist Bioscience) expressed candidates via cell‑free and Fc‑tagged mammalian systems.
• 4. Binding assays measured KD ≤1 µM and no off‑target CLEC12A binding.
• 5. 17 designs met all criteria for individual toxins.
• 6. Data and designs were released publicly for inspection.

证据

Claude generated 318,484 candidate minibinders in 64 hours

来源

211 designs tested

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17 binders

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binding in the single‑digit nanomolar KD range

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